Crystallization and preliminary X-ray crystallographic analysis of the tetracycline-degrading monooxygenase TetX2 from Bacteroides thetaiotaomicron

The flavin‐dependent monooxygenase TetX2 from Bacteroides thetaiotaomicron confers resistance against tetracyclines in aerobically grown Escherichia coli. TetX2 modifies several tetracycline antibiotics by regioselective hydroxylation of the substrates to 11a‐hydroxy‐tetracyclines. X‐ray diffraction data were collected from a native TetX2 crystal and a TetX2 crystal with incorporated selenomethionine to resolutions of 2.5 and 3.0 Å, respectively. The native crystal belonged to the triclinic space group P1, with unit‐cell parameters a = 68.55, b = 80.88, c = 87.53 Å, α = 111.09, β = 98.98, γ = 93.38°, whereas the selenomethionine‐labelled TetX2 crystal belonged to the monoclinic space group P21, with unit‐cell parameters a = 87.34, b = 68.66, c = 152.48 Å, β = 101.08°.