Identification and characterization of amylase-binding protein C from Streptococcus mitis NS51

Summary A substantial proportion of the streptococcal species found in dental plaque biofilms are able to interact with the abundant salivary enzyme α‐amylase. These streptococci produce proteins that specifically bind amylase. An important plaque species, Streptococcus mitis, secretes a 36‐kDa amylase‐binding protein into the extracellular milieu. Proteins precipitated from S. mitis NS51 cell culture supernatant by the addition of purified salivary amylase were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to a membrane, and a prominent 36‐kDa band was cut from the membrane and sequenced to yield the N‐terminal amino acid sequence DSQAQYSNGV. Searching the S. mitis genome sequence database revealed a single open reading frame containing this sequence, and the gene was amplified by the S. mitis genomic DNA polymerase chain reaction. The coding region of this open reading frame, designated amylase‐binding protein C (AbpC), was cloned into an Escherichia coli expression vector and the recombinant AbpC (rAbpC) was purified from the soluble fraction of the E. coli cell lysate. Purified AbpC was found to interact with immobilized amylase, confirming AbpC as a new streptococcal amylase‐binding protein.