Topological Relationship of Prophage λ to the Bacterial Chromosome in Lysogenic Cells

X irradiation converts bacterial sex factor DNA covalent circles to more slowly sedimenting forms according to first order kinetics. The rate of conversion is greater for sex factors carrying λ prophage than it is for nonlysogenized sex factors. The magnitude of the difference and the absence of cov...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proc. Nat. Acad. Sci. U. S. 65: 200-5(Jan 1970) 1970-01, Vol.65 (1), p.200-205
Hauptverfasser:	Freifelder, David, Meselson, Matthew
Format:	Artikel
Sprache:	eng
Schlagworte:	BACTERIA Bacterial chromosomes BACTERIOPHAGES BACTERIOPHAGES/lambda, effects on DNA of x-irradiated Escherichia coli Biological Sciences: Genetics Centrifugation, Density Gradient Chromosomes, Bacterial Circles Coliphages DNA DNA, Viral - radiation effects ESCHERICHIA COLI Fall lines INFECTIONS Irradiation Lysogeny Models, Biological Molecular weight Molecules N28800 -Life Sciences-Radiation Effects on Microorganisms Prophages RADIATION EFFECTS Radiation Genetics SCHIZOMYCOTA/Escherichia coli, radiation effects on DNA of, effects of prophage lambda infection on x Sex factor Sex Factors X RADIATION X RADIATION/effects on DNA of Escherichia coli, effects of prophage lambda infection on
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	X irradiation converts bacterial sex factor DNA covalent circles to more slowly sedimenting forms according to first order kinetics. The rate of conversion is greater for sex factors carrying λ prophage than it is for nonlysogenized sex factors. The magnitude of the difference and the absence of covalent circular DNA from the product show that the prophage DNA is linearly inserted into the sex factor, as predicted by Campbell's model for lysogenization.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.65.1.200