Fabrication of nanoscale “curtain rods” for DNA curtains using nanoimprint lithography

The authors have developed a new lithographically based patterning process which significantly increases the throughput of experiments which probe how repair proteins scan DNA molecules for errors. In this process, nanoscale barriers are formed to interrupt the flow of a lipid bilayer in which DNA i...

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Veröffentlicht in:Journal of vacuum science & technology. A, Vacuum, surfaces, and films Vacuum, surfaces, and films, 2009-11, Vol.27 (6), p.3095-3098
Hauptverfasser: Fazio, T. A., Visnapuu, M., Greene, E. C., Wind, S. J.
Format: Artikel
Sprache:eng
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Zusammenfassung:The authors have developed a new lithographically based patterning process which significantly increases the throughput of experiments which probe how repair proteins scan DNA molecules for errors. In this process, nanoscale barriers are formed to interrupt the flow of a lipid bilayer in which DNA is tethered to proteins in the bilayer. The barriers trap the DNA, which is then stretched out by hydrodynamic flow, resulting in the formation of “DNA curtains.” Nanoimprint lithography is used to facilitate massively parallel data collection for protein diffusion experiments on DNA.
ISSN:1071-1023
0734-2101
2166-2746
1520-8567
2166-2754
DOI:10.1116/1.3259951