Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma

Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma

AIM: To evaluate the role of thymidine phosphorylase (TP) in cholangiocarcinoma using small interfering RNA (siRNA). METHODS: A human cholangiocarcinoma-derived cell line KKU-M139, which has a naturally high level of endogenous TP, had TP expression transiently knocked down using siRNA. Cell growth,...

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Veröffentlicht in:World journal of gastroenterology : WJG 2010-04, Vol.16 (13), p.1631-1638
Hauptverfasser: Thanasai, Jongkonnee, Limpaiboon, Temduang, Jearanaikoon, Patcharee, Sripa, Banchob, Pairojkul, Chawalit, Tantimavanich, Srisurang, Miwa, Masanao
Format: Artikel
Sprache:eng
Schlagworte:
Antimetabolites, Antineoplastic - pharmacology
Apoptosis
Bile Duct Neoplasms - drug therapy
Carcinoma, Squamous Cell - drug therapy
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cholangiocarcinoma - drug therapy
Endothelium, Vascular - pathology
Fluorouracil - therapeutic use
Humans
Neoplasm Invasiveness
Neovascularization, Pathologic
Original
RNA, Small Interfering - metabolism
Thymidine Phosphorylase - metabolism
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Zusammenfassung:AIM: To evaluate the role of thymidine phosphorylase (TP) in cholangiocarcinoma using small interfering RNA (siRNA). METHODS: A human cholangiocarcinoma-derived cell line KKU-M139, which has a naturally high level of endogenous TP, had TP expression transiently knocked down using siRNA. Cell growth, migration, in vitro angiogenesis, apoptosis, and cytotoxicity were assayed in TP knockdown and wild-type cell lines. RESULTS: TP mRNA and protein expression were decreased by 87.1% ± 0.49% and 72.5% ± 3.2%, resp...
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v16.i13.1631