Acoustophoretic Synchronization of Mammalian Cells in Microchannels

We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectiv...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-04, Vol.82 (7), p.3094-3098
Hauptverfasser:	Thévoz, Patrick, Adams, Jonathan D, Shea, Herbert, Bruus, Henrik, Soh, H. Tom
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Biological and medical sciences Cell Cycle Cell cycle, cell proliferation Cell Line, Tumor Cell physiology Chemistry Exact sciences and technology Flow Cytometry Fundamental and applied biological sciences. Psychology Mammals Mice Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Molecular and cellular biology Propidium - chemistry Radiation Ultrasonic technology Ultrasonics
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Zusammenfassung:	We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G1 phase synchrony (∼84%) and throughput (3 × 106 cells/h per microchannel).
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac100357u