In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror

We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and...

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Veröffentlicht in:	Optics letters 2009-08, Vol.34 (15), p.2309-2311
Hauptverfasser:	PIYAWATTANAMETHA, Wibool, COCKER, Eric D, BURNS, Laurie D, BARRETTO, Robert P. J, JUNG, Juergen C, RA, Hyejun, SOLGAARD, Olav, SCHNITZER, Mark J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Biological and medical sciences Brain - blood supply Brain - cytology Capillaries - cytology Computer-Aided Design Electronics Equipment Design Equipment Failure Analysis Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Lenses Mice Micro- and nanoelectromechanical devices (mems/nems) Micro-Electrical-Mechanical Systems - instrumentation Microscopy, Fluorescence, Multiphoton - instrumentation Miniaturization Reproducibility of Results Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices Sensitivity and Specificity Tissues, organs and organisms biophysics
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Zusammenfassung:	We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.
ISSN:	0146-9592 1539-4794
DOI:	10.1364/OL.34.002309