Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfab...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Optics letters 2006-07, Vol.31 (13), p.2018-2020
Hauptverfasser:	PIYAWATTANAMETHA, Wibool, BARRETTO, Robert P. J, KO, Tony H, FLUSBERG, Benjamin A, COCKER, Eric D, RA, Hyejun, LEE, Daesung, SOLGAARD, Olav, SCHNITZER, Mark J
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Computer Systems Electronics Equipment Design Equipment Failure Analysis Exact sciences and technology Fundamental areas of phenomenology (including applications) Image forming and processing Imaging and optical processing Lenses Lenses, prisms, and mirrors Mechanics Micro- and nanoelectromechanical devices (mems/nems) Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Miniaturization Optical elements, devices, and systems Optics Physics Reproducibility of Results Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices Sensitivity and Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.
ISSN:	0146-9592 1539-4794
DOI:	10.1364/OL.31.002018