Evaluation of Hepatitis C Virus Core Antigen Assays in Detecting Recombinant Viral Antigens of Various Genotypes

A single substitution within the hepatitis C virus core antigen sequence, A48T, which is observed in ~30% of individuals infected with genotype 2a virus, reduces the sensitivity of a commonly used chemiluminescence enzyme immunoassay. Quantitation of the antigen is improved by using a distinct antic...

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Veröffentlicht in:	Journal of Clinical Microbiology 2009-12, Vol.47 (12), p.4141-4143
Hauptverfasser:	Saeed, Mohsan, Suzuki, Ryosuke, Kondo, Madoka, Aizaki, Hideki, Kato, Takanobu, Mizuochi, Toshiaki, Wakita, Takaji, Watanabe, Haruo, Suzuki, Tetsuro
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Biological and medical sciences Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Genotype Hepacivirus - classification Hepacivirus - genetics Hepatitis C - diagnosis Hepatitis C - virology Hepatitis C Antigens - analysis Hepatitis C Antigens - chemistry Hepatitis C Antigens - genetics Hepatitis C virus Humans Immunoblotting Immunoenzyme Techniques Microbiology Miscellaneous Molecular Sequence Data Recombinant Proteins - analysis Recombinant Proteins - chemistry Recombinant Proteins - genetics Viral Core Proteins - analysis Viral Core Proteins - chemistry Viral Core Proteins - genetics Virology
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Zusammenfassung:	A single substitution within the hepatitis C virus core antigen sequence, A48T, which is observed in ~30% of individuals infected with genotype 2a virus, reduces the sensitivity of a commonly used chemiluminescence enzyme immunoassay. Quantitation of the antigen is improved by using a distinct anticore antibody with a different epitope.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.01437-09