A Primer for Morpholino Use in Zebrafish

Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used in zebrafish ( Danio rerio ). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.