Rapid SYBR Green I and Modified Probe Real-Time Reverse Transcription-PCR Assays Identify Influenza H1N1 Viruses and Distinguish between Pandemic and Seasonal Strains

A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid...

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Veröffentlicht in:	Journal of Clinical Microbiology 2009-11, Vol.47 (11), p.3714-3716
Hauptverfasser:	Yang, Ji-Rong, Lo, Je, Liu, Jian-Liang, Lin, Chao-Hua, Ho, Yu-Lin, Chen, Chun-Jung, Wu, Ho-Sheng, Liu, Ming-Tsan
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fundamental and applied biological sciences. Psychology Humans Influenza A Virus, H1N1 Subtype - classification Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - isolation & purification Influenza, Human - virology Microbiology Miscellaneous Oligonucleotide Probes - genetics Organic Chemicals - metabolism Reverse Transcriptase Polymerase Chain Reaction - methods Sensitivity and Specificity Staining and Labeling - methods Time Factors Viral Proteins - genetics Virology
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Zusammenfassung:	A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid and simultaneous typing and subtyping of influenza viruses.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.01646-09