Subunits α, β and γ of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes

Subunits α, β and γ of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes

Specific small interfering RNA (siRNA) constructs were used to test for the functional relation of subunits α, β, and γ of the epithelial Na + channel (ENaC) to the hypertonicity-induced cation channel (HICC) in confluent rat hepatocytes. In current-clamp recordings, hypertonic stress (300 → 400 mos...

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Veröffentlicht in:Pflügers Archiv 2008-03, Vol.455 (6), p.1089-1095
Hauptverfasser: Plettenberg, Sandra, Weiss, Eike C., Lemor, Robert, Wehner, Frank
Format: Artikel
Sprache:eng
Schlagworte:
Algorithms
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Size - drug effects
Cells, Cultured
Electrophysiology
Epithelial Sodium Channels - physiology
Hepatocytes - physiology
Human Physiology
Ion Channels
Microelectrodes
Microscopy, Acoustic
Molecular Medicine
Neurosciences
Rats
Receptors
Reverse Transcriptase Polymerase Chain Reaction
RNA, Small Interfering - pharmacology
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Zusammenfassung:Specific small interfering RNA (siRNA) constructs were used to test for the functional relation of subunits α, β, and γ of the epithelial Na + channel (ENaC) to the hypertonicity-induced cation channel (HICC) in confluent rat hepatocytes. In current-clamp recordings, hypertonic stress (300 → 400 mosM) increased membrane conductance from 75.4 ± 9.4 to 91.1 ± 11.2 pS ( p  
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-007-0355-7