Reversible Switching of pRNA Activity on the DNA Packaging Motor of Bacteriophage phi29
This paper reports a reversible switching of the biological activity of an RNA molecule, packaging RNA (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29. The switching mechanism contains two components: (1) inhibition of pRNA by a short antisense DNA (asDNA) that...
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Veröffentlicht in: | Journal of the American Chemical Society 2008-12, Vol.130 (52), p.17684-17687 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | This paper reports a reversible switching of the biological activity of an RNA molecule, packaging RNA (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29. The switching mechanism contains two components: (1) inhibition of pRNA by a short antisense DNA (asDNA) that can bind to the 3′ end of the pRNA and inactivate the packaging motor; and (2) reactivation of pRNA by isothermal removal of asDNA from pRNA through a strand displacement strategy. The switching process can be repeated for multiple cycles and has been demonstrated by gel electrophoresis and a virion assembly assay. |
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ISSN: | 0002-7863 1520-5126 |
DOI: | 10.1021/ja806075d |