Reversible Switching of pRNA Activity on the DNA Packaging Motor of Bacteriophage phi29

This paper reports a reversible switching of the biological activity of an RNA molecule, packaging RNA (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29. The switching mechanism contains two components: (1) inhibition of pRNA by a short antisense DNA (asDNA) that...

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Veröffentlicht in:Journal of the American Chemical Society 2008-12, Vol.130 (52), p.17684-17687
Hauptverfasser: Ko, Seung Hyeon, Chen, Yi, Shu, Dan, Guo, Peixuan, Mao, Chengde
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Sprache:eng
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Zusammenfassung:This paper reports a reversible switching of the biological activity of an RNA molecule, packaging RNA (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29. The switching mechanism contains two components: (1) inhibition of pRNA by a short antisense DNA (asDNA) that can bind to the 3′ end of the pRNA and inactivate the packaging motor; and (2) reactivation of pRNA by isothermal removal of asDNA from pRNA through a strand displacement strategy. The switching process can be repeated for multiple cycles and has been demonstrated by gel electrophoresis and a virion assembly assay.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja806075d