Urinary bladder smooth muscle engineered from adipose stem cells and a three dimensional synthetic composite

Abstract Human adipose stem cells were cultured in smooth muscle inductive media and seeded into synthetic bladder composites to tissue engineer bladder smooth muscle. 85:15 Poly-lactic-glycolic acid bladder dome composites were cast using an electropulled microfiber luminal surface combined with an...

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Veröffentlicht in:Biomaterials 2009-07, Vol.30 (19), p.3259-3270
Hauptverfasser: Jack, Gregory S, Zhang, Rong, Lee, Min, Xu, Yuhan, Wu, Ben M, Rodríguez, Larissa V
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Sprache:eng
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Zusammenfassung:Abstract Human adipose stem cells were cultured in smooth muscle inductive media and seeded into synthetic bladder composites to tissue engineer bladder smooth muscle. 85:15 Poly-lactic-glycolic acid bladder dome composites were cast using an electropulled microfiber luminal surface combined with an outer porous sponge. Cell-seeded bladders expressed smooth muscle actin, myosin heavy chain, calponinin, and caldesmon via RT-PCR and immunoflourescence. Nude rats ( n = 45) underwent removal of half their bladder and repair using: (i) augmentation with the adipose stem cell-seeded composites, (ii) augmentation with a matched acellular composite, or (iii) suture closure. Animals were followed for 12 weeks post-implantation and bladders were explanted serially. Results showed that bladder capacity and compliance were maintained in the cell-seeded group throughout the 12 weeks, but deteriorated in the acellular scaffold group sequentially with time. Control animals repaired with sutures regained their baseline bladder capacities by week 12, demonstrating a long-term limitation of this model. Histological analysis of explanted materials demonstrated viable adipose stem cells and increasing smooth muscle mass in the cell-seeded scaffolds with time. Tissue bath stimulation demonstrated smooth muscle contraction of the seeded implants but not the acellular implants after 12 weeks in vivo . Our study demonstrates the feasibility and short term physical properties of bladder tissue engineered from adipose stem cells.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2009.02.035