A strip-shield improves the efficiency of a solenoid coil in probes for high-field solid-state NMR of lossy biological samples

A strip-shield inserted between a high inductance double-tuned solenoid coil and the glass tube containing the sample improves the efficiency of probes used for high-field solid-state NMR experiments on lossy aqueous samples of proteins and other biopolymers. A strip-shield is a coil liner consistin...

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Veröffentlicht in:Journal of magnetic resonance (1997) 2009-09, Vol.200 (1), p.74-80
Hauptverfasser: Wu, Chin H., Grant, Christopher V., Cook, Gabriel A., Park, Sang Ho, Opella, Stanley J.
Format: Artikel
Sprache:eng
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Zusammenfassung:A strip-shield inserted between a high inductance double-tuned solenoid coil and the glass tube containing the sample improves the efficiency of probes used for high-field solid-state NMR experiments on lossy aqueous samples of proteins and other biopolymers. A strip-shield is a coil liner consisting of thin copper strips layered on a PTFE (polytetrafluoroethylene) insulator. With lossy samples, the shift in tuning frequency is smaller, the reduction in Q, and RF-induced heating are all significantly reduced when the strip-shield is present. The performance of 800MHz 1H/15N and 1H/13C double-resonance probes is demonstrated on aqueous samples of membrane proteins in phospholipid bilayers.
ISSN:1090-7807
1096-0856
DOI:10.1016/j.jmr.2009.06.004