The Cytotoxicity of Microglass Fibers on Alveolar Macrophages of Fischer 344 Rats Evaluated by Cell Magnetometry, Cytochemisry and Morphology

Objectives:The toxicity of microglass fibers (MG), one of the man-made mineral fibers, has not been sufficiently evaluated. The aim of the current study was to evaluate the cytotoxicity of MG in vitro. Methods:Alveolar macrophages were obtained from the bronchoalveolar lavage of male F344/N rats. Th...

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Veröffentlicht in:Environmental Health and Preventive Medicine 2005-03, Vol.10 (2), p.111-119
Hauptverfasser: Shinji, Hisako, Watanabe, Mitsuyasu, Kudo, Yuichiro, Niitsuya, Masato, Tsunoda, Masashi, Satoh, Toshihiko, Sakai, Yasuhiro, Kotani, Makoto, Aizawa, Yoshiharu
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Sprache:eng
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Zusammenfassung:Objectives:The toxicity of microglass fibers (MG), one of the man-made mineral fibers, has not been sufficiently evaluated. The aim of the current study was to evaluate the cytotoxicity of MG in vitro. Methods:Alveolar macrophages were obtained from the bronchoalveolar lavage of male F344/N rats. The macrophages were exposed to MG at concentrations of 0, 40, 80, 160 and 320 μg/ml. The effects of MG on the macrophages were examined by cell magnetometry, LDH assay and morphological observation. Results:In the cell magnetometry experiment, a significant delay of relaxation (the reduction of remanent magnetic field strength) was observed in the cells treated with 160 and 320 μg/ml of MG in a dose-dependent manner. A significant increase in LDH release was also observed in the cells with 160 and 320 μg/ml in a dose-dependent manner. Changes in the cytoskeleton were observed after exposure to MG, by immunofluorescent microscopy using an α-tubulin antibody. Conclusions:The cytotoxicity of MG on alveolar macrophages was demonstrated with cell magnetometry. The mechanism of the toxic effects of MG was related to cytoskeleton damage.
ISSN:1342-078X
1347-4715
DOI:10.1007/bf02898001