Significance of Bcl-xL in human colon carcinoma

To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma. Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the Bcl-xL gene was measured by immunohistochemistry method. The correlation of Bcl-xL expression w...

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Veröffentlicht in:World journal of gastroenterology : WJG 2008-05, Vol.14 (19), p.3069-3073
Hauptverfasser: Zhang, You-Li, Pang, Li-Qun, Wu, Ying, Wang, Xiao-Yan, Wang, Chong-Qiang, Fan, Yu
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Sprache:eng
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Zusammenfassung:To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma. Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the Bcl-xL gene was measured by immunohistochemistry method. The correlation of Bcl-xL expression with clinical index was evaluated. After human colon cancer cell line HT29 was transfected with Bcl-xL small interfering RNA (siRNA), the anchorage-independent growth of cancer cells was detected by colony formation in soft agar and invasion ability of cancer cells was determined by a transwell model. The Bcl-xL expression was higher in cancerous tissue samples than in normal tissue samples (38.78 +/-11.36 vs 0.89 +/- 0.35, P < 0.001), and was associated with the pathological grade, lymph node metastasis and Duke's stage of colorectal carcinoma. Transfection with Bcl-xL siRNA inhibited the colony formation and invasion ability of human colon cancer cell line HT29 in vitro. Bcl-xL gene plays an important role in carcinogenesis of human colorectal carcinoma and is associated with malignant biological behaviors of human colorectal carcinoma.
ISSN:1007-9327
DOI:10.3748/wjg.14.3069