Protein Architecture of the Human Kinetochore Microtubule Attachment Site

Protein Architecture of the Human Kinetochore Microtubule Attachment Site

Chromosome segregation requires assembly of kinetochores on centromeric chromatin to mediate interactions with spindle microtubules and control cell-cycle progression. To elucidate the protein architecture of human kinetochores, we developed a two-color fluorescence light microscopy method that meas...

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Veröffentlicht in:Cell 2009-05, Vol.137 (4), p.672-684
Hauptverfasser: Wan, Xiaohu, O'Quinn, Ryan P., Pierce, Heather L., Joglekar, Ajit P., Gall, Walt E., DeLuca, Jennifer G., Carroll, Christopher W., Liu, Song-Tao, Yen, Tim J., McEwen, Bruce F., Stukenberg, P. Todd, Desai, Arshad, Salmon, E.D.
Format: Artikel
Sprache:eng
Schlagworte:
CELLCYLE
Cytoskeletal Proteins
DNA-Binding Proteins - metabolism
HeLa Cells
Humans
Kinetochores - chemistry
Kinetochores - metabolism
Metaphase
Microscopy, Fluorescence
Microtubule-Associated Proteins - metabolism
Microtubules - chemistry
Microtubules - metabolism
Nuclear Proteins
PROTEINS
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Zusammenfassung:Chromosome segregation requires assembly of kinetochores on centromeric chromatin to mediate interactions with spindle microtubules and control cell-cycle progression. To elucidate the protein architecture of human kinetochores, we developed a two-color fluorescence light microscopy method that measures average label separation, Delta, at
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2009.03.035