Translocation of a Thioether-Bridged Azurin Peptide Fragment via the Sec Pathway in Lactococcus lactis

This study demonstrates for the first time that a thioether-containing peptide, an azurin fragment, can be translocated via the Sec pathway. This methyl-lanthionine was introduced by the nisin modification enzymes. The Sec pathway can therefore be a successful alternative for those cyclized peptides...

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Veröffentlicht in:	Applied and Environmental Microbiology 2009-06, Vol.75 (11), p.3800-3802
Hauptverfasser:	Kuipers, Anneke, Rink, Rick, Moll, Gert N
Format:	Artikel
Sprache:	eng
Schlagworte:	Azurin - metabolism Bacterial Proteins - metabolism Enzymes Lactococcus lactis Lactococcus lactis - metabolism Membrane Transport Proteins - metabolism Microbiology Peptide Fragments - metabolism Peptides Physiology Protein Transport Studies
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	This study demonstrates for the first time that a thioether-containing peptide, an azurin fragment, can be translocated via the Sec pathway. This methyl-lanthionine was introduced by the nisin modification enzymes. The Sec pathway can therefore be a successful alternative for those cyclized peptides that are inefficiently transported via NisT.
ISSN:	0099-2240 1098-5336 1098-6596
DOI:	10.1128/AEM.00341-09