Endothelin and sex hormones modulate the fibronectin synthesis by cultured human skin scleroderma fibroblasts

Objective:To evaluate the influence of endothelin-1 (ET-1) and sex hormones on cell proliferation and extracellular matrix (ECM) synthesis (ie, fibronectin, laminin) by cultured normal and scleroderma (SSc) human skin fibroblasts (FBs).Methods:Primary cultures of FBs were treated with ET-1 and sex h...

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Veröffentlicht in:	Annals of the rheumatic diseases 2009-04, Vol.68 (4), p.599-602
Hauptverfasser:	Soldano, S, Montagna, P, Villaggio, B, Parodi, A, Gianotti, G, Sulli, A, Seriolo, B, Secchi, M E, Cutolo, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Basic and Translational Research Biological and medical sciences Biopsy Blotting, Western - methods Case-Control Studies Cell culture Cell growth Cell proliferation Cell Proliferation - drug effects Cells, Cultured Collagen Diseases of the osteoarticular system Drug Synergism Endothelin 1 Endothelin-1 - pharmacology Estradiol - pharmacology Experiments Extracellular matrix Extracellular Matrix - metabolism Female Fibroblasts Fibroblasts - drug effects Fibroblasts - metabolism Fibronectin Fibronectins - biosynthesis Gonadal Steroid Hormones - pharmacology Growth factors Hormones Humans Immunocytochemistry Immunoglobulins Immunohistochemistry Laminin Male Medical sciences Menopause Pathogenesis Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Scleroderma Scleroderma, Localized - metabolism Sex hormones Skin Skin - metabolism Statistical analysis Statistics, Nonparametric Studies Testosterone Testosterone - pharmacology
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Zusammenfassung:	Objective:To evaluate the influence of endothelin-1 (ET-1) and sex hormones on cell proliferation and extracellular matrix (ECM) synthesis (ie, fibronectin, laminin) by cultured normal and scleroderma (SSc) human skin fibroblasts (FBs).Methods:Primary cultures of FBs were treated with ET-1 and sex hormones (17β-oestradiol or testosterone) for 24 h. Cell growth was analysed by methiltetrazolium salt test, ECM synthesis was evaluated by immunocytochemistry and western blot, both at 24 h.Results:In normal FBs, ET-1 and 17β-oestradiol, as well as their combination, increased cell growth (p
ISSN:	0003-4967 1468-2060
DOI:	10.1136/ard.2008.097378