In Vitro Effects of Gamma Radiation from ⁶⁰Co and ¹³⁷Cs on Plasmid DNA

In Vitro Effects of Gamma Radiation from ⁶⁰Co and ¹³⁷Cs on Plasmid DNA

The effects of gamma radiation from ⁶⁰Co and ¹³⁷Cs on DNA in aqueous solution are studied experimentally. Using an improved plasmid purification protocol and improved electrophoretic gel analysis techniques provided results with relatively small uncertainties. The results are compared with both theo...

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Veröffentlicht in:Journal of biological physics 2007-04, Vol.33 (2), p.155-160
Hauptverfasser: Milian, F. M, Gouveia, A. N, Gual, M. R, Echeimberg, J. O, Arruda-Neto, J. D. T, Garcia, F, Schenberg, A. C. G, Vicente, E. J, Rodriguez, O, Guzman, F, Deppman, A
Format: Artikel
Sprache:eng
Schlagworte:
Aqueous solutions
Double-strand breaks
Gamma effects
ionizing radiation
pBSKS + DNA
Research Paper
Scavenger-derived radicals
Single-strand breaks
Studies
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Zusammenfassung:The effects of gamma radiation from ⁶⁰Co and ¹³⁷Cs on DNA in aqueous solution are studied experimentally. Using an improved plasmid purification protocol and improved electrophoretic gel analysis techniques provided results with relatively small uncertainties. The results are compared with both theoretical and experimental results. In particular, the results obtained here are discussed in the light of recent discussion on supposed differences of the effects induced by gamma radiation from ⁶⁰Co and ¹³⁷Cs. We find that the effects of both types of gamma radiation are similar.
ISSN:0092-0606
1573-0689
DOI:10.1007/s10867-007-9050-3