Regulatory networks define phenotypic classes of human stem cell lines

Human stem cells: surfing the PluriNet Hundreds of different human cell lines are grouped under the catch-all term 'stem cells'. They can be from embryos, fetuses or adults. And they can be pluripotent — able to produce a broad range of cells — or fated to produce a limited repertoire of c...

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Veröffentlicht in:Nature 2008-09, Vol.455 (7211), p.401-405
Hauptverfasser: Müller, Franz-Josef, Laurent, Louise C., Kostka, Dennis, Ulitsky, Igor, Williams, Roy, Lu, Christina, Park, In-Hyun, Rao, Mahendra S., Shamir, Ron, Schwartz, Philip H., Schmidt, Nils O., Loring, Jeanne F.
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Zusammenfassung:Human stem cells: surfing the PluriNet Hundreds of different human cell lines are grouped under the catch-all term 'stem cells'. They can be from embryos, fetuses or adults. And they can be pluripotent — able to produce a broad range of cells — or fated to produce a limited repertoire of cell types. Müller et al . set out to establish a 'stem cell diagnostic' to bring order to the characterization and classification of human stem cells, based on a database of transcriptional profiles derived from more than 150 cell samples. Bioinformatic analyses revealed that pluripotent stem cell lines share many properties and all possess a characteristic protein–protein network, dubbed 'PluriNet'. Other cell types, including brain-derived neural stem cell lines, are much more diverse. These results offer a new strategy for classifying stem cells and support the idea that pluripotency and self-renewal are under tight control by specific molecular networks. Stem cells are defined as self-renewing cell populations that can differentiate into multiple distinct cell types. However, hundreds of different human cell lines from embryonic, fetal and adult sources have been called stem cells, even though they range from pluripotent cells—typified by embryonic stem cells, which are capable of virtually unlimited proliferation and differentiation—to adult stem cell lines, which can generate a far more limited repertoire of differentiated cell types. The rapid increase in reports of new sources of stem cells and their anticipated value to regenerative medicine 1 , 2 has highlighted the need for a general, reproducible method for classification of these cells 3 . We report here the creation and analysis of a database of global gene expression profiles (which we call the ‘stem cell matrix’) that enables the classification of cultured human stem cells in the context of a wide variety of pluripotent, multipotent and differentiated cell types. Using an unsupervised clustering method 4 , 5 to categorize a collection of ∼150 cell samples, we discovered that pluripotent stem cell lines group together, whereas other cell types, including brain-derived neural stem cell lines, are very diverse. Using further bioinformatic analysis 6 we uncovered a protein–protein network (PluriNet) that is shared by the pluripotent cells (embryonic stem cells, embryonal carcinomas and induced pluripotent cells). Analysis of published data showed that the PluriNet seems to be a common characteristic of pluripo
ISSN:0028-0836
1476-4687
1476-4679
DOI:10.1038/nature07213