Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA

We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites. Isolates from the same outbreak had identical double-stranded RNA sequences, suggesting that this technique...

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Veröffentlicht in:	Emerging infectious diseases 2001-01, Vol.7 (1), p.141-145
Hauptverfasser:	Xiao, L, Limor, J, Bern, C, Lal, A A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cattle Cryptosporidiosis Cryptosporidium parvum Cryptosporidium parvum - classification Cryptosporidium parvum - genetics Genotype Humans Molecular Sequence Data RNA RNA, Double-Stranded - chemistry RNA, Protozoan - chemistry
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Zusammenfassung:	We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites. Isolates from the same outbreak had identical double-stranded RNA sequences, suggesting that this technique may be useful for tracking Cryptosporidium infection sources.
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid0701.010121