Stress-strain measurements and viscoelastic response of blood vessels cryopreserved by vitrification
To gain increased insight into thermo-mechanical phenomena during cryopreservation, tensile stress relaxation experiments were conducted on vitrified blood vessels (vitreous in Latin means Glassy), and the results compared with various viscoelastic models. Using a recently presented device, isotherm...
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Veröffentlicht in: | Annals of biomedical engineering 2007-12, Vol.35 (12), p.2077-2086 |
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Sprache: | eng |
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Zusammenfassung: | To gain increased insight into thermo-mechanical phenomena during cryopreservation, tensile stress relaxation experiments were conducted on vitrified blood vessels (vitreous in Latin means Glassy), and the results compared with various viscoelastic models. Using a recently presented device, isothermal stress-relaxation results were obtained for a bovine carotid artery model, permeated with the cryoprotectant cocktail VS55 and a reference solution of 7.05 M DMSO. After a rapidly applied tensile strain, experimental results display exponential decay of stress with time; the stress at a given time increases with decreasing specimen temperature. Among the viscoelastic models investigated, the Williams-Landel-Ferry model was found to best-fit the variation of the stress relaxation data with temperature, while a Maxwell-Weichert model is used to represent the exponential decay of the stress with time. Blood vessel properties were found to dominate at temperatures above roughly -100 degrees C, while the properties of the cryoprotectant dominate below this temperature. A suitably defined steady-state viscosity displayed a similar behavior for both cryoprotectants, when normalized with respect to the cryoprotectant glass transition temperature. |
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ISSN: | 0090-6964 1573-9686 1521-6047 |
DOI: | 10.1007/s10439-007-9372-0 |