Quantification of sunitinib in human plasma by high-performance liquid chromatography–tandem mass spectrometry

A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sunitinib in human plasma. Sample preparation involved a liquid–liquid extraction by the addition of 0.2 mL of plasma with 4.0 mL tert-butyl-methyl-ether extraction solution containing 25 ng/mL of...

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Veröffentlicht in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-10, Vol.874 (1), p.84-88
Hauptverfasser: Minkin, Patton, Zhao, Ming, Chen, Zhaoyuan, Ouwerkerk, Jan, Gelderblom, Hans, Baker, Sharyn D.
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Sprache:eng
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Zusammenfassung:A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sunitinib in human plasma. Sample preparation involved a liquid–liquid extraction by the addition of 0.2 mL of plasma with 4.0 mL tert-butyl-methyl-ether extraction solution containing 25 ng/mL of the internal standard clozapine. Separation of compounds was achieved on a C 18 (50 mm × 2.1 mm i.d., 3.5 μm) analytical column using a mobile phase consisting of acetonitrile/H 20 (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.150 mL/min for 3 min. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves in human plasma were generated over the range of 0.2–500 ng/mL with values for the coefficient of determination of >0.9950. Within- and between day precision and accuracy were ≤10%. The method was applied to the quantitation of sunitinib in plasma samples from a patient receiving daily oral therapy with sunitinib.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.09.007