Live Cell Surface Labeling with Fluorescent Ag Nanocluster Conjugates

Live Cell Surface Labeling with Fluorescent Ag Nanocluster Conjugates

DNA‐encapsulated silver clusters are readily conjugated to proteins and serve as alternatives to organic dyes and semiconductor quantum dots. Stable and bright on the bulk and single molecule levels, Ag nanocluster fluorescence is readily observed when staining live cell surfaces. Being significantl...

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Veröffentlicht in:Photochemistry and photobiology 2008-11, Vol.84 (6), p.1435-1439
Hauptverfasser: Yu, Junhua, Choi, Sungmoon, Richards, Chris I., Antoku, Yasuko, Dickson, Robert M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Survival
Cells
Heparan sulfate
Labeling
Mass spectrometry
Microscopy, Fluorescence
Molecular weight
Nanostructures - chemistry
Proteins
Silver - chemistry
Studies
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Beschreibung
Zusammenfassung:DNA‐encapsulated silver clusters are readily conjugated to proteins and serve as alternatives to organic dyes and semiconductor quantum dots. Stable and bright on the bulk and single molecule levels, Ag nanocluster fluorescence is readily observed when staining live cell surfaces. Being significantly brighter and more photostable than organics and much smaller than quantum dots with a single point of attachment, these nanomaterials offer promising new approaches for bulk and single molecule biolabeling.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.2008.00434.x