Efficient Ends-Out Gene Targeting In Drosophila

In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-pos...

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Veröffentlicht in:	Genetics (Austin) 2008-09, Vol.180 (1), p.703-707
Hauptverfasser:	Huang, Juan, Zhou, Wenke, Watson, Annie M, Jan, Yuh-Nung, Hong, Yang
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified Chromosomes Chromosomes - ultrastructure Cloning, Molecular Crosses, Genetic crossing DNA repair Drosophila - genetics Drosophila melanogaster ends-out targeting vectors Female Females Gene Targeting genetic markers Genetic Techniques Genetic Vectors insect genetics Labor intensive Male Mice Models, Genetic negative selection markers plasmid vectors Polymerase Chain Reaction Recombination, Genetic Systems development
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Beschreibung
Zusammenfassung:	In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.
ISSN:	0016-6731 1943-2631 1943-2631
DOI:	10.1534/genetics.108.090563