Denitration of L-type calcium channel

Tyrosine nitration results in altered function of selective proteins, including human smooth muscle L-type calcium channel, hCa v1.2b. We report here that Ca v1.2 is also subject to “denitration”. Cell lysates from activated macrophage-like cell line, RAW264.7 cells, reversed peroxynitrite-induced n...

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Veröffentlicht in:	FEBS letters 2008-09, Vol.582 (20), p.3033-3036
Hauptverfasser:	Kang, Minho, Akbarali, Hamid I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Calcium Channels, L-Type - genetics Calcium Channels, L-Type - metabolism Cell Line Humans Ion channel Mice Muscle, Smooth - metabolism Nitrates - metabolism Nitration Nitric oxide Phosphorylation Smooth muscle Src kinase Tyrosine Tyrosine - analogs & derivatives Tyrosine - biosynthesis
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Zusammenfassung:	Tyrosine nitration results in altered function of selective proteins, including human smooth muscle L-type calcium channel, hCa v1.2b. We report here that Ca v1.2 is also subject to “denitration”. Cell lysates from activated macrophage-like cell line, RAW264.7 cells, reversed peroxynitrite-induced nitration of the carboxy terminus of Ca v1.2 in a 1D gel assay. Tyrosine phosphorylation of the calcium channel by c-src kinase was blocked by nitration but reversed by pretreatment with RAW264.7 cell lysates. These findings indicate that denitration may be a physiological mechanism to restore cellular excitability during inflammation.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2008.07.042