Optical Imaging of Bacterial Infection in Living Mice Using a Fluorescent Near-Infrared Molecular Probe

An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive...

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Veröffentlicht in:Journal of the American Chemical Society 2006-12, Vol.128 (51), p.16476-16477
Hauptverfasser: Leevy, W. Matthew, Gammon, Seth T, Jiang, Hua, Johnson, James R, Maxwell, Dustin J, Jackson, Erin N, Marquez, Manuel, Piwnica-Worms, David, Smith, Bradley D
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Sprache:eng
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Zusammenfassung:An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0665592