New and Sensitive Assay for Determining Pseudomonas aeruginosa Metallo-Beta-Lactamase Resistance to Imipenem

We present an imipenem lysate metallo-beta-lactamase (MBL) functional assay. This assay eliminates false-positive results due to the bactericidal effects of EDTA, can be performed with inexpensive reagents available in most laboratories, and is as accurate as the MBL Etest. It is appropriate for bot...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-05, Vol.46 (5), p.1870-1872
Hauptverfasser:	Tan, Jingyuan, Pitout, Johann D.D, Guttman, David S
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Bacteriology beta-Lactam Resistance beta-Lactamases - biosynthesis Biological and medical sciences Fundamental and applied biological sciences. Psychology Imipenem - pharmacology Microbial Sensitivity Tests - methods Microbiology Miscellaneous Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - enzymology
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Beschreibung
Zusammenfassung:	We present an imipenem lysate metallo-beta-lactamase (MBL) functional assay. This assay eliminates false-positive results due to the bactericidal effects of EDTA, can be performed with inexpensive reagents available in most laboratories, and is as accurate as the MBL Etest. It is appropriate for both high-accuracy screens and laboratories in developing countries with limited resources.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.02175-07