Diagnostic performance indices for immunofluorescent tests and enzyme immunoassays of leishmaniasis sera from northern and north-eastern Brazil

A total of 341 sera were screened for anti-Leishmania IgA, IgG, and IgM antibodies by immunofluorescent (IF) tests and enzyme immunoassay (ELISA). Altogether, 292 of the sera originated from patients with clinically as well as parasitologically diagnosed (positive lesion imprint or the Montenegro sk...

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Veröffentlicht in:	Bulletin of the World Health Organization 1990, Vol.68 (1), p.39-43
Hauptverfasser:	GUIMARAES, M. C. S, CELESTE, B. J, FRANCO, E. L
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Biological and medical sciences Brazil - epidemiology Child Child, Preschool Female Fluorescent Antibody Technique - standards Humans Immunoenzyme Techniques - standards Infant Investigative techniques, diagnostic techniques (general aspects) Leishmaniasis - diagnosis Leishmaniasis - epidemiology Male Medical sciences Middle Aged Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Sensitivity and Specificity
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Zusammenfassung:	A total of 341 sera were screened for anti-Leishmania IgA, IgG, and IgM antibodies by immunofluorescent (IF) tests and enzyme immunoassay (ELISA). Altogether, 292 of the sera originated from patients with clinically as well as parasitologically diagnosed (positive lesion imprint or the Montenegro skin test) cutaneous leishmaniasis; 49 of the sera were from controls from the same base population. In terms of diagnostic performance, the ELISAs for IgG and IgM yielded indices of diagnostic utility, and the positive predictive value for the IgG-ELISA was 94.6%. A remarkably high specificity (100%) was obtained with the IgA-IF test, but its sensitivity was very low.
ISSN:	0042-9686 1564-0604