Inhibition of immunoglobulin E response to Japanese cedar pollen allergen (Cry j 1) in mice by DNA immunization: different outcomes dependent on the plasmid DNA inoculation method

Summary To develop a new immunotherapy for Japanese cedar (Cryptomeria japonica; CJ) pollinosis, we evaluated the use of DNA immunization by inoculating mice with plasmid DNA encoding Cry j 1 as a CJ pollen major allergen (pCACJ1). Repeated intramuscular (i.m.) inoculation of BALB/c mice with pCACJ1...

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Veröffentlicht in:Immunology 2000-02, Vol.99 (2), p.179-186
Hauptverfasser: Toda, M., Sato, H., Takebe, Y., Taniguchi, Y., Saito, S., Inouye, S., Takemori, T., Sakaguchi, M.
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Sprache:eng
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Zusammenfassung:Summary To develop a new immunotherapy for Japanese cedar (Cryptomeria japonica; CJ) pollinosis, we evaluated the use of DNA immunization by inoculating mice with plasmid DNA encoding Cry j 1 as a CJ pollen major allergen (pCACJ1). Repeated intramuscular (i.m.) inoculation of BALB/c mice with pCACJ1 produced anti‐Cry j 1 antibody responses, which were predominately of the immunoglobulin G2a (IgG2a) type. Furthermore, this inoculation suppressed immunoglobulin E (IgE) and IgG1 antibody responses to subsequent alum‐precipitated Cry j 1 injections. Splenic T cells isolated from mice inoculated with pCACJ1 i.m. secreted interferon‐γ (IFN‐γ), but not interleukin (IL)‐4, in vitro upon stimulation with Cry j 1 as well as with p277–288, a peptide corresponding to the T‐cell epitope of Cry j 1. In contrast, inoculation of BALB/c mice with pCACJ1 by gene gun injection caused response predominantly of the IgG1 type, and enhanced production of anti‐Cry j 1 IgE antibodies to subsequent alum‐precipitated Cry j 1 injections. Splenic T cells isolated from pCACJ1‐innoculated mice by gene gun injection secreted both IFN‐γ and IL‐4 in vitro, upon stimulation with Cry j 1 as well as with p277–288. These findings suggest that i.m. inoculation with pCACJ1 effectively elicits Cry j 1‐specific T helper 1 (Th1)‐type immune responses, resulting in inhibition of the IgE response to Cry j 1.
ISSN:0019-2805
1365-2567
DOI:10.1046/j.1365-2567.2000.00935.x