Connective Tissue Growth Factor: A Cysteine-Rich Mitogen Secreted by Human Vascular Endothelial Cells Is Related to the SRC-Induced Immediate Early Gene Product CEF-10

Human umbilical vein endothelial (HUVE) cells have been previously reported to express the genes for the A and B chains of PDGF and to secrete PDGF-related factors into culture media. Antihuman PDGF IgG affinity chromatography was used to purify PDGF-related activity from HUVE cell-conditioned media...

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Veröffentlicht in:	The Journal of cell biology 1991-09, Vol.114 (6), p.1285-1294
Hauptverfasser:	Bradham, Douglass M., Igarashi, Atsuyuki, Potter, Robert L., Grotendorst, Gary R.
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 cells Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Antibodies Base Sequence Binding, Competitive Biological and medical sciences Biological Assay Cell growth Cell Line Cells Cloning, Molecular Complementary DNA Connective Tissue Growth Factor Culture Media Endothelial cells Endothelium, Vascular - metabolism Endothelium, Vascular - physiology Fundamental and applied biological sciences. Psychology Gels Gene Library genes Genes, src Growth Substances - genetics Growth Substances - isolation & purification Growth Substances - metabolism Humans Immediate-Early Proteins Intercellular Signaling Peptides and Proteins Kinetics Macromolecular Substances Mice mitogens Molecular Sequence Data Molecules NIH 3T3 cells Platelet-Derived Growth Factor - genetics Platelet-Derived Growth Factor - isolation & purification Platelet-Derived Growth Factor - metabolism Platelets Protein Biosynthesis Protein hormones. Growth factors. Cytokines Proteins Receptors, Cell Surface - metabolism Receptors, Platelet-Derived Growth Factor Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Restriction Mapping RNA, Messenger - genetics Transcription, Genetic Umbilical Veins
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Zusammenfassung:	Human umbilical vein endothelial (HUVE) cells have been previously reported to express the genes for the A and B chains of PDGF and to secrete PDGF-related factors into culture media. Antihuman PDGF IgG affinity chromatography was used to purify PDGF-related activity from HUVE cell-conditioned media. Immunoblot analysis of the affinity-purified proteins with anti-PDGF IgG and antibodies specific for the A or B chain peptides of PDGF combined with chemotactic and mitogenic assays revealed that the major PDGF immunorelated molecule secreted by HUVE cells is a monomer of ~36-38 kD and that
ISSN:	0021-9525 1540-8140
DOI:	10.1083/jcb.114.6.1285