Determination of Glycosyltransferase Specificities for the Escherichia coli O111 O Antigen by a Generic Approach

We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-ant...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-02, Vol.74 (4), p.1294-1298
Hauptverfasser:	Stevenson, Gordon, Dieckelmann, Manuela, Reeves, Peter R
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens Bacteriology Biochemistry Biological and medical sciences Cloning, Molecular E coli Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genetics and Molecular Biology Glucosyltransferases - genetics Glucosyltransferases - metabolism Microbiology Models, Biological Multigene Family O Antigens - genetics O Antigens - metabolism Oligonucleotides - genetics Species Specificity Substrate Specificity
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Beschreibung
Zusammenfassung:	We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.02660-07