The kinetics of the lysis of Bacterium coli by glycine

The lysis of Bacterium coli suspensions brought about by glycine shows the following characteristics: (1) There is a latent period of 2 hr., followed by a rapid lysis reaching a maximum in about 8 hr. (2) The extent of the lysis is independent of the dilution of the bacterial suspension over a wide range. (3) The extent of the lysis increases with the glycine concentration up to 10M, but is approaching a limit at this concentration. (4) The lysis is negligible below pH 5 and above pH 10, and shows a maximum rate in the region of pH 6–5–8–5. (5) The rate of lysis has a very high temperature coefficient (Q10 of the order of 5).