Buffered Osmium Tetroxide ( OsO4) Fixation for Cytological and Feulgen Microspectrophotometric Studies of Human Rectal Polyps

Buffered Osmium Tetroxide ( OsO4) Fixation for Cytological and Feulgen Microspectrophotometric Studies of Human Rectal Polyps

Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also...

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Veröffentlicht in:Journal of biophysical and biochemical cytology 1955-09, Vol.1 (5), p.385-390
Hauptverfasser: Leuchtenberger, Cecilie, Doolin, Paul F., Kutsakis, Anne H.
Format: Artikel
Sprache:eng
Schlagworte:
Buffers
Cell Nucleus
Chromatin
Coloring Agents
Cytoplasm
DNA
Epithelial cells
Histological Techniques
Humans
Inclusion bodies
Liver
Mucosa
Neoplasms
Nuclear membrane
Old Medline
Osmium
Osmium Tetroxide
Polyps
Rectal Neoplasms
Rectum
Rosaniline Dyes
Staining and Labeling
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Zusammenfassung:Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO(4)-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei.
ISSN:0095-9901
0021-9525
1540-8140
DOI:10.1083/jcb.1.5.385