Fluorescence Resonance Energy Transfer (FRET) Microscopy Imaging of Live Cell Protein Localizations

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resoluti...

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Veröffentlicht in:	The Journal of cell biology 2003-03, Vol.160 (5), p.629-633
Hauptverfasser:	Sekar, Rajesh Babu, Periasamy, Ammasi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cells Cellular biology Emission spectra Energy Energy transfer Eukaryotic Cells - cytology Eukaryotic Cells - metabolism Fluorescence Fluorescence Resonance Energy Transfer - methods Fluorescence Resonance Energy Transfer - trends Fluorescent Dyes Green Fluorescent Proteins Humans Image Processing, Computer-Assisted - methods Image Processing, Computer-Assisted - trends Imaging Luminescent Proteins Microscopy Microscopy, Fluorescence - methods Microscopy, Fluorescence - trends Mini-Review Mini-Reviews Molecular imaging NIH 3T3 cells Proteins Proteins - metabolism Receptors Scientific imaging Spectroscopy
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Beschreibung
Zusammenfassung:	The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
ISSN:	0021-9525 1540-8140
DOI:	10.1083/jcb.200210140