Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses

Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AF...

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Veröffentlicht in:	Journal of Clinical Microbiology 2007-12, Vol.45 (12), p.4048-4050
Hauptverfasser:	Kalpoe, J.S, Templeton, K.E, Horrevorts, A.M, Endtz, H.P, Kuijper, E.J, Bernards, A.T, Klaassen, C.H.W
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Bacteriology Biological and medical sciences Cluster Analysis DNA Fingerprinting DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Epidemiology Female Fundamental and applied biological sciences. Psychology Humans Male Microbiology Middle Aged Miscellaneous Molecular Epidemiology - methods Nocardia - classification Nocardia - genetics Nocardia - isolation & purification Nocardia farcinica Nocardia Infections - epidemiology Nocardia Infections - microbiology Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique
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Zusammenfassung:	Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.00932-07