Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima

Class II α‐mannosidase cleaves off α‐1,2‐, α‐1,3‐ and α‐1,6‐mannose residues. In this paper, the crystallization and preliminary X‐ray analysis of cytosolic class II α‐mannosidase from Thermotoga maritima (TM1851), a family 38 glycoside hydrolase, is described. The crystal of recombinant TM1851 belongs to the C‐­centred monoclinic space group C2, with unit‐cell parameters a = 244.7, b = 87.4, c = 166.6 Å, β = 124.7°. X‐ray diffraction data were collected to a resolution of 2.9 Å.