Cyclic Nucleotide-Gated Channels on the Flagellum Control Ca2+ Entry into Sperm
Cyclic nucleotide-gated (CNG) channels are key elements of cGMP- and cAMP-signaling pathways in vertebrate photoreceptor cells and in olfactory sensory neurons, respectively. These channels form heterooligomeric complexes composed of at least two distinct subunits (α and β). The α subunit of cone ph...
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| Veröffentlicht in: | The Journal of cell biology 1998-07, Vol.142 (2), p.473-484 |
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| creator | Wiesner, Burkhard Weiner, Jocelyn Middendorff, Ralf Hagen, Volker Kaupp, U. Benjamin Weyand, Ingo |
| description | Cyclic nucleotide-gated (CNG) channels are key elements of cGMP- and cAMP-signaling pathways in vertebrate photoreceptor cells and in olfactory sensory neurons, respectively. These channels form heterooligomeric complexes composed of at least two distinct subunits (α and β). The α subunit of cone photoreceptors is also present in mammalian sperm. Here we identify one short and several long less abundant transcripts of β subunits in testis. The α and β subunits are expressed in a characteristic temporal and spatial pattern in sperm and precursor cells. In mature sperm, the α subunit is observed along the entire flagellum, whereas the short β subunit is restricted to the principal piece of the flagellum. These findings suggest that different forms of CNG channels coexist in the flagellum. Confocal microscopy in conjunction with the Ca2+ indicator Fluo-3 shows that the CNG channels serve as a Ca2+ entry pathway that responds more sensitively to cGMP than to cAMP. Assuming that CNG channel subtypes differ in their Ca2+ permeability, dissimilar localization of α and β subunits may give rise to a pattern of Ca2+ microdomains along the flagellum, thereby providing the structural basis for control of flagellar bending waves. |
| doi_str_mv | 10.1083/jcb.142.2.473 |
| format | Article |
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Benjamin ; Weyand, Ingo</creator><creatorcontrib>Wiesner, Burkhard ; Weiner, Jocelyn ; Middendorff, Ralf ; Hagen, Volker ; Kaupp, U. Benjamin ; Weyand, Ingo</creatorcontrib><description>Cyclic nucleotide-gated (CNG) channels are key elements of cGMP- and cAMP-signaling pathways in vertebrate photoreceptor cells and in olfactory sensory neurons, respectively. These channels form heterooligomeric complexes composed of at least two distinct subunits (α and β). The α subunit of cone photoreceptors is also present in mammalian sperm. Here we identify one short and several long less abundant transcripts of β subunits in testis. The α and β subunits are expressed in a characteristic temporal and spatial pattern in sperm and precursor cells. In mature sperm, the α subunit is observed along the entire flagellum, whereas the short β subunit is restricted to the principal piece of the flagellum. These findings suggest that different forms of CNG channels coexist in the flagellum. Confocal microscopy in conjunction with the Ca2+ indicator Fluo-3 shows that the CNG channels serve as a Ca2+ entry pathway that responds more sensitively to cGMP than to cAMP. Assuming that CNG channel subtypes differ in their Ca2+ permeability, dissimilar localization of α and β subunits may give rise to a pattern of Ca2+ microdomains along the flagellum, thereby providing the structural basis for control of flagellar bending waves.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.142.2.473</identifier><identifier>PMID: 9679145</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Amino Acid Sequence ; Amino acids ; Aniline Compounds ; Animals ; Antibodies ; Base Sequence ; Calcium - metabolism ; Calcium Channels - chemistry ; Calcium Channels - genetics ; Calcium Channels - metabolism ; Cattle ; Cellular biology ; Cloning, Molecular ; Complementary DNA ; Cyclic AMP - metabolism ; Cyclic GMP - metabolism ; DNA Primers - genetics ; DNA, Complementary - genetics ; Flagella ; Fluorescence ; Fluorescent Dyes ; Gene Expression ; Immunohistochemistry ; Ion Transport ; Male ; Mammals ; Microscopy, Confocal ; Molecular Sequence Data ; Nucleotides ; Polymerase Chain Reaction ; Protein Conformation ; Reproduction ; Sperm Tail - metabolism ; Spermatozoa ; Testes ; Testis - metabolism ; Ungulates ; Xanthenes</subject><ispartof>The Journal of cell biology, 1998-07, Vol.142 (2), p.473-484</ispartof><rights>Copyright 1998 The Rockefeller University Press</rights><rights>Copyright Rockefeller University Press Jul 27, 1998</rights><rights>1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-d8aa078a093ac97eb29a86366fe6bc48374824ec30e0e6dc8e7984588d70f6653</citedby><cites>FETCH-LOGICAL-c362t-d8aa078a093ac97eb29a86366fe6bc48374824ec30e0e6dc8e7984588d70f6653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9679145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wiesner, Burkhard</creatorcontrib><creatorcontrib>Weiner, Jocelyn</creatorcontrib><creatorcontrib>Middendorff, Ralf</creatorcontrib><creatorcontrib>Hagen, Volker</creatorcontrib><creatorcontrib>Kaupp, U. Benjamin</creatorcontrib><creatorcontrib>Weyand, Ingo</creatorcontrib><title>Cyclic Nucleotide-Gated Channels on the Flagellum Control Ca2+ Entry into Sperm</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Cyclic nucleotide-gated (CNG) channels are key elements of cGMP- and cAMP-signaling pathways in vertebrate photoreceptor cells and in olfactory sensory neurons, respectively. These channels form heterooligomeric complexes composed of at least two distinct subunits (α and β). The α subunit of cone photoreceptors is also present in mammalian sperm. Here we identify one short and several long less abundant transcripts of β subunits in testis. The α and β subunits are expressed in a characteristic temporal and spatial pattern in sperm and precursor cells. In mature sperm, the α subunit is observed along the entire flagellum, whereas the short β subunit is restricted to the principal piece of the flagellum. These findings suggest that different forms of CNG channels coexist in the flagellum. Confocal microscopy in conjunction with the Ca2+ indicator Fluo-3 shows that the CNG channels serve as a Ca2+ entry pathway that responds more sensitively to cGMP than to cAMP. Assuming that CNG channel subtypes differ in their Ca2+ permeability, dissimilar localization of α and β subunits may give rise to a pattern of Ca2+ microdomains along the flagellum, thereby providing the structural basis for control of flagellar bending waves.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Aniline Compounds</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Base Sequence</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - chemistry</subject><subject>Calcium Channels - genetics</subject><subject>Calcium Channels - metabolism</subject><subject>Cattle</subject><subject>Cellular biology</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic GMP - metabolism</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>Flagella</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes</subject><subject>Gene Expression</subject><subject>Immunohistochemistry</subject><subject>Ion Transport</subject><subject>Male</subject><subject>Mammals</subject><subject>Microscopy, Confocal</subject><subject>Molecular Sequence Data</subject><subject>Nucleotides</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Conformation</subject><subject>Reproduction</subject><subject>Sperm Tail - metabolism</subject><subject>Spermatozoa</subject><subject>Testes</subject><subject>Testis - metabolism</subject><subject>Ungulates</subject><subject>Xanthenes</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtrGzEUhUVpSR23y-5aEFl0U8a9eowem0IZkrQQkkXatZA11_EYzcgdzQT876Nikz5WEpzDx3c5hLxjsGJgxOddWK-Y5Cu-klq8IAtWS6gMk_CSLAA4q2zN69fkPOcdAEgtxRk5s0pbJusFuWsOIXaB3s4hYpq6FqtrP2FLm60fBoyZpoFOW6RX0T9gjHNPmzRMY4q08fwTvSz_A-2GKdH7PY79G_Jq42PGt6d3SX5eXf5ovlU3d9ffm683VRCKT1VrvAdtPFjhg9W45tYbJZTaoFoHaYSWhksMAhBQtcGgtkbWxrQaNkrVYkm-HLn7ed1jG7B4-Oj2Y9f78eCS79y_ydBt3UN6dJwJATUrgI8nwJh-zZgn13c5lAv9gGnOzgCIoiBL8eK_4i7N41COKywN1tRFfEmqYymMKecRN88mDNzvmVyZyZWZHHdlptL_8Lf-c_u0S8nfH_NdntL4B6aY0VaJJz7Blqg</recordid><startdate>19980727</startdate><enddate>19980727</enddate><creator>Wiesner, Burkhard</creator><creator>Weiner, Jocelyn</creator><creator>Middendorff, Ralf</creator><creator>Hagen, Volker</creator><creator>Kaupp, U. Benjamin</creator><creator>Weyand, Ingo</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980727</creationdate><title>Cyclic Nucleotide-Gated Channels on the Flagellum Control Ca2+ Entry into Sperm</title><author>Wiesner, Burkhard ; Weiner, Jocelyn ; Middendorff, Ralf ; Hagen, Volker ; Kaupp, U. Benjamin ; Weyand, Ingo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-d8aa078a093ac97eb29a86366fe6bc48374824ec30e0e6dc8e7984588d70f6653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Aniline Compounds</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Base Sequence</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - chemistry</topic><topic>Calcium Channels - genetics</topic><topic>Calcium Channels - metabolism</topic><topic>Cattle</topic><topic>Cellular biology</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic GMP - metabolism</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>Flagella</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes</topic><topic>Gene Expression</topic><topic>Immunohistochemistry</topic><topic>Ion Transport</topic><topic>Male</topic><topic>Mammals</topic><topic>Microscopy, Confocal</topic><topic>Molecular Sequence Data</topic><topic>Nucleotides</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Conformation</topic><topic>Reproduction</topic><topic>Sperm Tail - metabolism</topic><topic>Spermatozoa</topic><topic>Testes</topic><topic>Testis - metabolism</topic><topic>Ungulates</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wiesner, Burkhard</creatorcontrib><creatorcontrib>Weiner, Jocelyn</creatorcontrib><creatorcontrib>Middendorff, Ralf</creatorcontrib><creatorcontrib>Hagen, Volker</creatorcontrib><creatorcontrib>Kaupp, U. 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Benjamin</au><au>Weyand, Ingo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclic Nucleotide-Gated Channels on the Flagellum Control Ca2+ Entry into Sperm</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1998-07-27</date><risdate>1998</risdate><volume>142</volume><issue>2</issue><spage>473</spage><epage>484</epage><pages>473-484</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>Cyclic nucleotide-gated (CNG) channels are key elements of cGMP- and cAMP-signaling pathways in vertebrate photoreceptor cells and in olfactory sensory neurons, respectively. These channels form heterooligomeric complexes composed of at least two distinct subunits (α and β). The α subunit of cone photoreceptors is also present in mammalian sperm. Here we identify one short and several long less abundant transcripts of β subunits in testis. The α and β subunits are expressed in a characteristic temporal and spatial pattern in sperm and precursor cells. In mature sperm, the α subunit is observed along the entire flagellum, whereas the short β subunit is restricted to the principal piece of the flagellum. These findings suggest that different forms of CNG channels coexist in the flagellum. Confocal microscopy in conjunction with the Ca2+ indicator Fluo-3 shows that the CNG channels serve as a Ca2+ entry pathway that responds more sensitively to cGMP than to cAMP. Assuming that CNG channel subtypes differ in their Ca2+ permeability, dissimilar localization of α and β subunits may give rise to a pattern of Ca2+ microdomains along the flagellum, thereby providing the structural basis for control of flagellar bending waves.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>9679145</pmid><doi>10.1083/jcb.142.2.473</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of cell biology, 1998-07, Vol.142 (2), p.473-484 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Amino acids Aniline Compounds Animals Antibodies Base Sequence Calcium - metabolism Calcium Channels - chemistry Calcium Channels - genetics Calcium Channels - metabolism Cattle Cellular biology Cloning, Molecular Complementary DNA Cyclic AMP - metabolism Cyclic GMP - metabolism DNA Primers - genetics DNA, Complementary - genetics Flagella Fluorescence Fluorescent Dyes Gene Expression Immunohistochemistry Ion Transport Male Mammals Microscopy, Confocal Molecular Sequence Data Nucleotides Polymerase Chain Reaction Protein Conformation Reproduction Sperm Tail - metabolism Spermatozoa Testes Testis - metabolism Ungulates Xanthenes |
| title | Cyclic Nucleotide-Gated Channels on the Flagellum Control Ca2+ Entry into Sperm |
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