Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis

We report the sequence of a cDNA clone that codes for the carboxy-terminal portion of the peroxisomal protein, acyl-CoA oxidase, from the yeast, Candida tropicalis. This is a newly identified acyl-CoA oxidase sequence, most likely a second allele of POX4. The cDNA clone was expressed by in vitro tra...

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Veröffentlicht in:The Journal of cell biology 1987-07, Vol.105 (1), p.247-250
Hauptverfasser: Small, Gillian M., Lazarow, Paul B.
Format: Artikel
Sprache:eng
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Zusammenfassung:We report the sequence of a cDNA clone that codes for the carboxy-terminal portion of the peroxisomal protein, acyl-CoA oxidase, from the yeast, Candida tropicalis. This is a newly identified acyl-CoA oxidase sequence, most likely a second allele of POX4. The cDNA clone was expressed by in vitro transcription followed by translation. The major product, a 43-kD protein, associated with isolated peroxisomes in an in vitro import assay. More than half of the peroxisome-associated protein was protected from added protease, implying that it was internalized within the organelle. These findings indicate that there is sufficient information in the carboxy-terminal portion of the protein to target it to peroxisomes.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.105.1.247