Reappearance of β-Adrenergic Receptors after Isoproterenol Treatment in Intact C6-Cells

The reappearance of β-adrenergic receptors in C6-glioma cells after desensitization with isoproterenol was studied using the antagonist [3H] CGP-12177. Reappearance had the following properties: (a) it occurred in intact cells only, (b) it was temperature dependent, (c) it required an Na+/ H+gradien...

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Veröffentlicht in:	The Journal of cell biology 1983-11, Vol.97 (5), p.1538-1543
Hauptverfasser:	Hertel, Cornelia, Staehelin, Matthys
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenergic beta-Antagonists - metabolism Adrenergic receptors Animals Binding sites Biological and medical sciences Cations - pharmacology Cell Line Cell membranes Cell physiology Cycloheximide - pharmacology Endocytosis Fundamental and applied biological sciences. Psychology Glioma - metabolism Inurement Isoproterenol - pharmacology Kinetics Ligands Lysosomes - metabolism Molecular and cellular biology Neuroglia Neurotransmission Propanolamines - metabolism Rats Receptors Receptors, Adrenergic, beta - drug effects Recycling Temperature Temperature dependence
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Beschreibung
Zusammenfassung:	The reappearance of β-adrenergic receptors in C6-glioma cells after desensitization with isoproterenol was studied using the antagonist [3H] CGP-12177. Reappearance had the following properties: (a) it occurred in intact cells only, (b) it was temperature dependent, (c) it required an Na+/ H+gradient, low intracellular Ca2+activity, and (d) it required ATP, and (e) intact lysosomes. The results suggest endocytosis and recycling of the β-adrenergic receptor after agonist treatment.
ISSN:	0021-9525 1540-8140
DOI:	10.1083/jcb.97.5.1538