Immunohistochemical Localization of Contractile Proteins in Limulus Striated Muscle

Limulus paramyosin and myosin were localized in the A bands of glycerinated Limulus striated muscle by the indirect horseradish peroxidase-labeled antibody and direct and indirect fluorescent antibody techniques. Localization of each protein in the A band varied with sarcomere length. Antiparamyosin...

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Veröffentlicht in:	The Journal of cell biology 1972-10, Vol.55 (1), p.221-235
Hauptverfasser:	Rhea J. C. Levine, Dewey, Maynard M., de Villafranca, George W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actomyosin - analysis Animals Antibodies Antibody Specificity Antigen-Antibody Reactions Binding Sites, Antibody Biological markers Brachyura Contractile proteins Fluorescent Antibody Technique Horseradish Marine Microscopy, Phase-Contrast Models, Biological Molecules Muscle Proteins - analysis Muscles Muscles - analysis Mustard Plant - enzymology Myofibrils Myosins - analysis Peroxidases - pharmacology Plants, Medicinal Precipitin Tests Rabbits - immunology Sarcomeres Solar fibrils Staining and Labeling Striated muscle
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Zusammenfassung:	Limulus paramyosin and myosin were localized in the A bands of glycerinated Limulus striated muscle by the indirect horseradish peroxidase-labeled antibody and direct and indirect fluorescent antibody techniques. Localization of each protein in the A band varied with sarcomere length. Antiparamyosin was bound at the lateral margins of the A bands in long (∼ 10.0 μ) and intermediate (∼ 7.0 μ) length sarcomeres, and also in a thin line in the central A bands of sarcomeres, 7.0-∼6.0 μ. Antiparamyosin stained the entire A bands of short sarcomeres (
ISSN:	0021-9525 1540-8140
DOI:	10.1083/jcb.55.1.221