Isolation of Ribosomes from Cysts of Entamoeba invadens

Isolation of Ribosomes from Cysts of Entamoeba invadens

A reproducible method for the preparation of 75S ribosomes from cysts of Entamoeba invadens is presented. The method depends on the inactivation of the cyst's ribonuclease by high levels of Bentonite. The ribosomes are found to be extremely sensitive to ribonuclease, and to be stabilized by the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of cell biology 1968-01, Vol.36 (1), p.45-51
Hauptverfasser: Morgan, Richard S., Slayter, Henry S., Weller, David L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bentonite
Calcium
Crystals
Cysts
Deoxyribonucleases - metabolism
Electron microscopes
Entamoeba - cytology
Entamoeba - metabolism
Entamoeba - ultrastructure
Glycogen - metabolism
Magnesium
Ribonucleoproteins
Ribosomes
Ribosomes - metabolism
Ribosomes - ultrastructure
RNA
Spectrophotometry, Ultraviolet
Trophozoites
Trophozoites - cytology
Trophozoites - ultrastructure
Ultracentrifugation
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A reproducible method for the preparation of 75S ribosomes from cysts of Entamoeba invadens is presented. The method depends on the inactivation of the cyst's ribonuclease by high levels of Bentonite. The ribosomes are found to be extremely sensitive to ribonuclease, and to be stabilized by the addition of manganous and calcium ions to the magnesium customarily employed. Reasons are given for equating these ribosomes with the particles of which the crystalline chromatoid bodies are made.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.36.1.45