The effect of thymidine on the duration of G1 in Chinese hamster cells

The effect of thymidine on the duration of G1 in Chinese hamster cells

The generation time of a Chinese hamster cell line was varied by the use of different lots of sera in the culture media. Analysis of the division waves following thymidine synchronization showed that lengthening of the generation time was a result of an increase in duration of the G(1) phase and tha...

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Veröffentlicht in:The Journal of cell biology 1967-10, Vol.35 (1), p.53-59
Hauptverfasser: Tobey, R A, Anderson, E C, Petersen, D F
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cricetinae
Culture Media
Culture Techniques
Female
Mitosis - drug effects
Thymidine - pharmacology
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Zusammenfassung:The generation time of a Chinese hamster cell line was varied by the use of different lots of sera in the culture media. Analysis of the division waves following thymidine synchronization showed that lengthening of the generation time was a result of an increase in duration of the G(1) phase and that thymidine treatment reduced the duration of G(1) back to its minimum value.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.35.1.53