Experimental herpetic keratitis in rabbit corneal organ cultures

An in-vitro method for maintaining rabbit corneal organ cultures for short periods of time is described. These cultures supported replication of strains of type 1 and 2 herpes simplex virus, and changes typical of herpetic infections of the cornea in vivo were observed by light microscopy, and by sc...

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Veröffentlicht in:	British journal of experimental pathology 1984-02, Vol.65 (1), p.41-49
Hauptverfasser:	PERCY, D. H, CREIGHTON, M. O, HATCH, L. A, DE CLERCQ, E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal viral diseases Animals Biological and medical sciences Cornea - ultrastructure Epithelium - ultrastructure herpes simplex virus Infectious diseases Keratitis, Dendritic - pathology Medical sciences Microscopy, Electron Microscopy, Electron, Scanning Organ Culture Techniques Rabbits Simplexvirus - physiology Vacuoles - ultrastructure Viral diseases Virus Replication
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Zusammenfassung:	An in-vitro method for maintaining rabbit corneal organ cultures for short periods of time is described. These cultures supported replication of strains of type 1 and 2 herpes simplex virus, and changes typical of herpetic infections of the cornea in vivo were observed by light microscopy, and by scanning and transmission electron microscopy. Infectious virus was readily recovered from inoculated corneal organ cultures. For practical, economic and humanitarian reasons, the full potential of this system warrants further investigation.
ISSN:	0007-1021