Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography

We describe a reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify six major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of 1 g. Waters Atlantis C 18 3 μm served as the stationary phase. The gradient was formed using ammonium acetate and methanol, containing 2% propan-2-ol. Detection is linear between 5 ng/mL and 500 ng/mL in plasma (5–1000 ng/mL in urine). The coefficient of variation for intra- and inter-day variation is