Analysis of Tear Glucose Concentration with Electrospray Ionization Mass Spectrometry

We have developed a mass spectrometry-based method that allows one to accurately determine the glucose concentration of tear fluid. We used a 1 μL micro-capillary to collect tear fluid from the tear meniscus with minimal irritation of the eye. We analyzed the 1 μL volume of collected tear fluid with...

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Veröffentlicht in:Journal of the American Society for Mass Spectrometry 2007-02, Vol.18 (2), p.332-336
Hauptverfasser: Taormina, Christopher R., Baca, Justin T., Asher, Sanford A., Grabowski, Joseph J., Finegold, David N.
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Sprache:eng
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Zusammenfassung:We have developed a mass spectrometry-based method that allows one to accurately determine the glucose concentration of tear fluid. We used a 1 μL micro-capillary to collect tear fluid from the tear meniscus with minimal irritation of the eye. We analyzed the 1 μL volume of collected tear fluid with liquid-chromatography electrospray ionization mass spectrometry with the use of D-glucose-6,6-d 2 as an internal standard. Repeated measurements and a recovery experiment on pooled, onion-induced tears showed that the analysis of the glucose in tears was precise (4% relative standard deviation) and provided 100% recovery. We found the tear glucose concentration of one fasting nondiabetic subject to be 13 to 51 μM while the onion-induced tear glucose concentration of a different nondiabetic subject to be 211 to 256 μM.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2006.10.002