Common molecular signature in SOD1 for both sporadic and familial amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron degenerative disease whose etiology and pathogenesis remain poorly understood. Most cases of ALS ([almost equal to]90%) are sporadic (SALS), occurring in the absence of genetic associations. Approximately 20% of familial ALS (FALS) ca...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2007-07, Vol.104 (30), p.12524-12529
Hauptverfasser: Gruzman, Arie, Wood, William L, Alpert, Evgenia, Prasad, M. Dharma, Miller, Robert G, Rothstein, Jeffery D, Bowser, Robert, Hamilton, Ronald, Wood, Troy D, Cleveland, Don W, Lingappa, Vishwanath R, Liu, Jian
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Sprache:eng
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Zusammenfassung:Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron degenerative disease whose etiology and pathogenesis remain poorly understood. Most cases of ALS ([almost equal to]90%) are sporadic (SALS), occurring in the absence of genetic associations. Approximately 20% of familial ALS (FALS) cases are due to known mutations in the copper, zinc superoxide dismutase (SOD1) gene. Molecular evidence for a common pathogenesis of SALS and FALS has remained elusive. Here we use covalent chemical modification to reveal an attribute of spinal cord SOD1 common to both SOD1-linked FALS and SALS, but not present in normal or disease-affected tissues from other neurodegenerative diseases, including Alzheimer's, Parkinson's, and Huntington's diseases and spinal muscular atrophy, a non-ALS motor neuron disease. Biotinylation reveals a 32-kDa, covalently cross-linked SOD1-containing protein species produced not only in FALS caused by SOD1 mutation, but also in SALS. These studies use chemical modification as a novel tool for the detection of a disease-associated biomarker. Our results identify a shared molecular event involving a known target gene and suggest a common step in the pathogenesis between SALS and FALS.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0705044104