Different pharmacological characteristics in L6 and C2C12 muscle cells and intact rat skeletal muscle for amylin, CGRP and calcitonin

1 We compared the ability of rat amylin, rat calcitonin gene‐related peptide (CGRP) and rat and salmon calcitonins to elevate cyclic AMP levels and to inhibit [U‐14C]‐glucose incorporation into glycogen in insulin‐stimulated intact rat soleus muscle and in two cell lines derived from rodent skeletal...

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Veröffentlicht in:British journal of pharmacology 1996-03, Vol.117 (5), p.847-852
Hauptverfasser: Pittner, Richard A., Wolfe‐Lopez, Deborah, Young, Andrew A., Beaumont, Kevin
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Sprache:eng
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Zusammenfassung:1 We compared the ability of rat amylin, rat calcitonin gene‐related peptide (CGRP) and rat and salmon calcitonins to elevate cyclic AMP levels and to inhibit [U‐14C]‐glucose incorporation into glycogen in insulin‐stimulated intact rat soleus muscle and in two cell lines derived from rodent skeletal muscle, L6 and C2C12 2 In intact soleus muscle, both amylin (EC50s of 0.7‐6.1 nM) and salmon calcitonin (EC50s of 0.5‐1.4 nM) were more potent than CGRP (EC50s of 5.6–15.8 nM) and were much more potent than rat calcitonin (EC50s of 50–137 nM) at stimulating cyclic AMP production, activating glycogen phosphorylase and inhibiting insulin‐stimulated [14C]‐glycogen formation. 3 In contrast, in both L6 and C2C12 cells, CGRP (EC50s of 0.042‐0.12 nM) stimulated cyclic AMP formation and inhibited insulin‐stimulated [U‐14C]‐glucose incorporation into glycogen approximately 1000 times more potently than amylin (EC50s 34–240 nM), while salmon calcitonin was without measurable effect. 4 There was a correlation between elevation of cyclic AMP and inhibition of insulin‐stimulated [U‐14C]‐glucose incorporation into glycogen evoked by these peptides in both intact muscle (r2 = 0.69, P
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.1996.tb15270.x